CBX3 antibody (AA 74-87)

Catalog No. ABIN337123

This Goat Polyclonal antibody specifically detects CBX3 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Hamster, Monkey, Bat and Pig.

Quick Overview for CBX3 antibody (AA 74-87) (ABIN337123)

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Hamster, Monkey, Bat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This CBX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CBX3 Antibody

Binding Specificity: AA 74-87

Specificity: Human CBX3. No cross-reactivity expected with HP1-alpha and HP1-beta. NP_009207.2 and NP_057671.2 represent same protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum (100%) Turkey, Chicken, Platypus, Lizard, Xenopus, Frog (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EAFLNSQKAGKEKD from an internal region of human CBX3 (NP_009207.2, NP_057671.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum (100%), Turkey, Chicken, Platypus, Lizard, Xenopus, Frog (93%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CBX3

Target: CBX3 (Chromobox Homolog 3 (CBX3))

Alternative Name: CBX3 / HP1 Gamma

Background: Name/Gene ID: CBX3

Synonyms: CBX3, Chromobox homolog 3, HP1Hs-gamma, Heterochromatin-like protein 1, Modifier 2 protein, HP1-GAMMA, Chromobox protein homolog 3, HECH, HP1 gamma, HP1 gamma homolog

Gene ID: 11335

NCBI Accession: NP_009207, NP_057671

UniProt: Q13185