OGG1 antibody (AA 282-297)

Catalog No. ABIN337126

This Goat Polyclonal antibody specifically detects OGG1 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for OGG1 antibody (AA 282-297) (ABIN337126)

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This OGG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-OGG1 Antibody

Binding Specificity: AA 282-297

Specificity: Mouse OGG1.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Mouse (100%), Rat (88%), Hamster (88%), Elephant (88%), Panda (88%), Bovine (88%), Human (81%), Gorilla (81%), Monkey (81%), Dog (81%), Horse (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-HPKTSQAKGPSPLANK from an internal region of mouse OGG1 (NP_035087.2). Percent identity with other species by BLAST analysis: Mouse (100%), Rat (88%), Hamster (88%), Elephant (88%), Panda (88%), Bovine (88%), Human (81%), Gorilla (81%), Monkey (81%), Dog (81%), Horse (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000), WB (1:128000, 0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for OGG1

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Alternative Name: OGG1

Background: Name/Gene ID: OGG1

Synonyms: OGG1, 8-oxoguanine DNA glycosylase, AP lyase, HMMH, N-glycosylase/DNA lyase, HOGG1, OGH1, OGG1 type 1f, MMH, MUTM

Gene ID: 4968

NCBI Accession: NP_035087

UniProt: O15527

Pathways: DNA Damage Repair