GDF15 antibody (AA 286-299)

Catalog No. ABIN337140

The Goat Polyclonal anti-GDF15 antibody (ABIN337140) specifically detects GDF15 in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for GDF15 antibody (AA 286-299) (ABIN337140)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GDF15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GDF15 Antibody

Binding Specificity: AA 286-299

Specificity: Human GDF15.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QKTDTGVSLQTYDD from the C-terminus of human GDF15 (NP_004855.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset (93%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:2000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GDF15

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15

Background: Name/Gene ID: GDF15
Family: TGF beta

Synonyms: GDF15, MIC1, MIC-1, NAG-1, NRG-1, NSAID-regulated gene 1 protein, PLAB, NSAID-activated gene 1 protein, Placental TGF-beta, PTGFB, GDF-15, PTGF-beta

Gene ID: 9518

NCBI Accession: NP_004855

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interactome