GOT2 antibody (AA 295-306)

Catalog No. ABIN337180

This Goat Polyclonal antibody specifically detects GOT2 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Horse, Monkey and Hamster.

Quick Overview for GOT2 antibody (AA 295-306) (ABIN337180)

Target: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Reactivity: Human, Horse, Monkey, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This GOT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GOT2 Antibody

Binding Specificity: AA 295-306

Specificity: Human GOT2.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Hamster, Panda, Horse, Opossum (100%) Mouse, Rat, Bovine, Pig, Catfish, Salmon, Zebrafish (92%) Elephant, Turkey, Chicken, Platypus, Xenopus, Pufferfish, Poplar, Castor bean (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide CKDADEAKRVES from an internal region of human GOT2 (NP_002071.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Hamster, Panda, Horse, Opossum (100%), Mouse, Rat, Bovine, Pig, Catfish, Salmon, Zebrafish (92%), Elephant, Turkey, Chicken, Platypus, Xenopus, Pufferfish, Poplar, Castor bean (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GOT2

Target: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Alternative Name: GOT2

Background: Name/Gene ID: GOT2

Synonyms: GOT2, FABP-1, MitAAT, Transaminase A, FABPpm, Fatty acid-binding protein, KAT4, KATIV, Kynurenine aminotransferase IV, MAspAT

Gene ID: 2806

NCBI Accession: NP_002071

UniProt: P00505

Pathways: Monocarboxylic Acid Catabolic Process