PEDF antibody (AA 196-347)

Catalog No. ABIN337197

The Mouse Monoclonal anti-PEDF antibody (ABIN337197) specifically detects PEDF in WB, IHC, ELISA, ICC, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for PEDF antibody (AA 196-347) (ABIN337197)

Target: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PEDF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PEDF Antibody

Binding Specificity: AA 196-347

Specificity: Recognizes human GST-PEDF fusion protein with a MW of ~46kD.

Purification: Protein A purified

Immunogen: Recombinant human GST-PEDF fusion protein. The sequence corresponding to aa196-347 of human PEDF tagged with GST on the N-terminal was expressed in E. coli. GST-PEDF is about 46kD on SDS-PAGE.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, ICC, IHC, IHC-Fr, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid freeze-thaw cycles.

Target Details for PEDF

Target: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Alternative Name: SERPINF1 / PEDF

Background: Name/Gene ID: SERPINF1

Synonyms: SERPINF1, EPC-1, OI12, PEDF, Serpin F1, PIG35, OI6

Gene ID: 5176

UniProt: P36955