PEDF antibody (N-Term)

Catalog No. ABIN6739671

This Rabbit Polyclonal antibody specifically detects PEDF in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Pig, Dog, Guinea Pig, Horse, Sheep and Bat.

Quick Overview for PEDF antibody (N-Term) (ABIN6739671)

Target: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Reactivity: Human, Mouse, Rat, Pig, Dog, Guinea Pig, Horse, Sheep, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEDF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PEDF Antibody

Binding Specificity: N-Term

Specificity: Human SERPINF1 / PEDF

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Sheep (86%) Horse, Pig, Guinea pig (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human SERPINF1 (P36955, NP_002606). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Bat (100%), Gibbon (93%), Galago (92%), Marmoset, Mouse, Sheep, Bovine (86%), Opossum, Platypus (81%), Elephant, Horse, Pig, Guinea pig (80%), Rat (75%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (4 - 8 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 4-8 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS buffer with 2 % sucrose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PEDF

Target: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Alternative Name: SERPINF1 / PEDF

Background: Name/Gene ID: SERPINF1

Synonyms: SERPINF1, EPC-1, OI12, PEDF, Serpin F1, PIG35, OI6

Gene ID: 5176

NCBI Accession: NP_002606

UniProt: P36955