TRKB antibody (AA 1-429)

Catalog No. ABIN337266

The Rabbit Polyclonal anti-TRKB antibody (ABIN337266) specifically detects TRKB in WB, IHC and IHC (p). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for TRKB antibody (AA 1-429) (ABIN337266)

Target: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRKB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TRKB Antibody

Binding Specificity: AA 1-429

Specificity: Recognizes rat TrkB, Mr 145kD, as well as an additional protein, Mr ~90kD, which is likely a TrkB degradation product. Species sequence homology: mouse 97 % and human 88 %.

Purification: Protein A purified

Immunogen: The entire extracellular domain (corresponding to residues 1-429) of the rat TrkB receptor, expressed in COS cells.

Type of Immunogen: Cells

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 0.5-2 μg/mL detected TrkB in mouse brain membrane protein preparations.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1M Tris-glycine,  pH 7.4, 0.15M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TRKB

Target: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Alternative Name: NTRK2 / TRKB

Background: Name/Gene ID: NTRK2
Subfamily: Trk/Ror
Family: Protein Kinase

Synonyms: NTRK2, gp145-TrkB, Trk-B, TRKB, Tropomyosin-related kinase B, TrkB tyrosine kinase, Tyrosine kinase receptor B

Gene ID: 4915

UniProt: Q16620

Pathways: RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport