MIF antibody (AA 103-115)

Catalog No. ABIN337308

The Goat Polyclonal anti-MIF antibody (ABIN337308) specifically detects MIF in WB, IHC, ELISA and IHC (p). The antibody is reactive with Human and Monkey samples.

Quick Overview for MIF antibody (AA 103-115) (ABIN337308)

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This MIF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MIF Antibody

Binding Specificity: AA 103-115

Specificity: Human MIF.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Mouse, Rat, Sheep, Elephant, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Xenopus (92%) Hamster (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-NAANVGWNNSTFA from the C-terminus of human MIF (NP_002406.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Mouse, Rat, Sheep, Elephant, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Xenopus (92%), Hamster (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000), WB (0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Alternative Name: MIF

Background: Name/Gene ID: MIF

Synonyms: MIF, GLIF, L-dopachrome tautomerase, L-dopachrome isomerase, MMIF, Phenylpyruvate tautomerase

Gene ID: 4282

NCBI Accession: NP_002406

UniProt: P14174

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling