PPP2R3A antibody (AA 1073-1085)

Catalog No. ABIN337311

The Goat Polyclonal anti-PPP2R3A antibody (ABIN337311) specifically detects PPP2R3A in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human, Mouse, Horse, Rabbit, Pig and Monkey samples.

Quick Overview for PPP2R3A antibody (AA 1073-1085) (ABIN337311)

Target: PPP2R3A (Protein Phosphatase 2, Regulatory Subunit B'', alpha (PPP2R3A))

Reactivity: Human, Mouse, Horse, Rabbit, Pig, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP2R3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PPP2R3A Antibody

Binding Specificity: AA 1073-1085

Specificity: Human PPP2R3A. This antibody is expected to recognise both reported isoforms (NP_002709.2, NP_871626.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Horse, Rabbit, Pig, Lizard (100%) Rat, Hamster, Bat, Bovine, Opossum, Turkey, Chicken (92%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QKDVENDGPEPSD from an internal region of human PPP2R3A (NP_002709.2, NP_871626.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Horse, Rabbit, Pig, Lizard (100%), Rat, Hamster, Bat, Bovine, Opossum, Turkey, Chicken (92%), Dog (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:16000), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PPP2R3A

Target: PPP2R3A (Protein Phosphatase 2, Regulatory Subunit B'', alpha (PPP2R3A))

Alternative Name: PPP2R3A / PR130

Background: Name/Gene ID: PPP2R3A

Synonyms: PPP2R3A, PR130, PR72, PPP2R3, PP2A, subunit B, R3 isoform

Gene ID: 5523

NCBI Accession: NP_002709, NP_871626

UniProt: Q06190

Pathways: PI3K-Akt Signaling