Heat Shock Protein Hsp90 beta (hsp90b) antibody

Antigen: Heat Shock Protein Hsp90 beta (hsp90b)

Clonality: Monoclonal (MBH90B)

Host: Mouse

Reactivity: Human, Mouse (Murine), Cow (Bovine)

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN343707

Additional Information

Alternative name: Hsp90 beta

Immunogen: Peptide corresponding to the EEVHHGEEEVEC sequence within N-terminal part of humanHsp90.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: MBH90B

Description: Hsp90 beta (heat shock protein 90 beta) is a constitutively expressed isoform of Hsp90, oneof the most abundant chaperones in the cytosol of eukaryotic cells. Hsp90 interacts withvarious proteins, including protein kinases and transcription factors, and either facilitatestheir stabilization and activation or directs them for proteasomal degradation. Hsp90 thusaffects multiple signaling pathways and biological processes and modulation of this singletarget offers the prospect of simultaneous intervence to various key points of oncogenictransformation. Hsp90 operates as a dimer in a conformational cycle driven by ATP bindingand hydrolysis.

Specificity: The antibody MBH90B recognizes the EEVHHG epitope within the N-terminal part of Hsp90beta an ubiquitously expressed protein with calculated Mw of 83.3 kDa, however, migratingas a 90 kDa band under reducing SDS-PAGE conditions.

Application Details

Application Notes: Immunoprecipitation - Recommended dilution: 1 µg/ml. Western Blotting - Recommended dilution: 1 µg/ml. Immunocytochemistry - Recommended dilution: 1 µg/ml

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Signaling, Heat Shock Proteins, Cancer

Restrictions: For Research Use only

Images

Western blotting analysis of MBH90B reactivity with various cell lines and recombinant Hsp90Alpha and Hsp90Beta protein.