Ubinuclein 1 (UBN1) antibody

Antigen: Ubinuclein 1 (UBN1)

Synonyms: VT, VT4, AA673476, AW124741, MGC102206, 1110029L11Rik, 2610108L02Rik, ubn1

Clonality: Monoclonal (UBN1-02)

Host: Mouse

Reactivity: Human

Application: Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN343714

Additional Information

Alternative name: Ubinuclein 1

Immunogen: Recombinant protein corresponding to amino acids 1-190 of ubinuclein 1.

Format: Purified

Isotype: IgG  (Matching secondary antibodies)

Clone: UBN1-02

Description: Ubinuclein 1 (UBN1) is a ubiquitously expressed evolutionarily conserved protein whichbinds to proliferation-promoting genes that are repressed by formation ofsenescence-associated heterochromatin foci (SAHF). Ubinuclein 1 associates with varioustranscription factors and with histone methyltransferase activity, is indispensable for SAHFformation and appears to be a regulator of senescence. Although in most cells ubinuclein islocalized to the nucleus, in cells forming tight junctions it is recruited to the cell adhesioncomplexes, dependently on the cell density.

Specificity: The antibody UBN1-02 recognizes N-terminal part of ubinuclein 1 (UBN1), a widelyexpressed nuclear and adhesion complex protein. Western blotting analysis reveals UBN1as a 165 kDa band.

Application Details

Application Notes: Western Blotting - Recommended dilution: 1-5 µg/ml. Positive control: HeLa cell line

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Western