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CD81 antibody (CD81 Molecule) (FITC)

Details for Product anti-CD81 Antibody No. ABIN343722, Supplier: Log in to see
Antigen
  • CVID6
  • S5.7
  • Tapa-1
  • TAPA1
  • Tapa1
  • TSPAN28
  • Tspan28
Reactivity
Human, Cat (Feline), Rabbit
216
169
31
30
29
18
18
18
14
5
3
2
Host
Mouse
160
94
47
27
10
5
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD81 antibody is conjugated to FITC
45
39
31
8
7
7
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
285
110
106
84
71
65
46
38
34
34
11
4
3
3
3
2
1
1
1
Supplier
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Immunogen MOLT-4 (human T-ALL cell line)
Clone M38
Isotype IgG1
Specificity The antibody M38 reacts with CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.
Characteristics The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
Purification Purified from ascites by protein-A affinity chromatography.
Alternative Name CD81 (CD81 Antibody Abstract)
Background CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells -, e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µL reagent / 100 µL of whole blood or 10^6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Product cited in: Escola, Kleijmeer, Stoorvogel, Griffith, Yoshie, Geuze: "Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20121-7, 1998

Imai, Kakizaki, Nishimura, Yoshie: "Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 3, pp. 1229-39, 1995

Imai, Yoshie et al.: "C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 6470-81, 1994

Fukudome, Furuse, Imai, Nishimura, Takagi, Hinuma, Yoshie et al.: "Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen ..." in: Journal of virology, Vol. 66, Issue 3, pp. 1394-401, 1992