Peptidyl Arginine Deiminase, Type IV (PADI4) antibody
| Antigen |
|
| Synonyms |
Pdi4, PAD, PAD4, PDI4, PDI5, PADI5 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
|
|
Conjugate
|
Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
|
|
Application
|
 Alternatives ELISA, Immunoprecipitation (IP), Western Blotting (WB)
Western Blotting (WB) (33), Immunofluorescence (IF) (22), ELISA (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunohistochemistry (IHC) (7), Immunoprecipitation (IP) (7), Flow Cytometry (FACS) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
|
| Catalog no. |
ABIN347070 |
| Quantity |
200 µl (Variants) |
| Price |
573.54 $ Plus shipping costs $45.00
|
|
Bulk discount
|
| Shipping to |
|
| Availability |
Will be delivered in 5 to 6 Business Days |
|
Alternative name
|
Peptidylarginine deiminase type IV (PAD-4)
|
|
Immunogen
|
A15 residue synthetic peptide derived from C-terminal domain
|
|
Format
|
Antiserum
|
|
Specificity
|
Antiserum reacts specifically with 74 kDa of PAD4 protein from human, mouse and rat. Cross Reactivities: There is no cross reaction with the other isoforms
|
|
Application Notes
|
Optimal dilutions should be determined by the end user.The following are guidelines only: ELISA : 1/1 000 to 1/5 000. Immunohistochimie : 1/50 to 1/100. Immunoprecipitation : 1/20 to 1/100. Western Blot : 1/200 to 1/2 000
|
|
Buffer
|
Lyophilized from 200 µl of serum. Preservative: None.
|
|
Storage
|
Short term storage: +4°C. Long term storage: -20°C
|
|
Restrictions
|
For Research Use only
|
|
|
Nakashima, Hagiwara, Ishigami et al.: "Molecular characterization of peptidylarginine deiminase in HL-60 cells induced by retinoic acid and 1alpha,25-dihydroxyvitamin D(3)." in: The Journal of biological chemistry, Vol. 274, Issue 39, pp. 27786-92, 1999 (PubMed).
Wang, Wysocka, Sayegh et al.: "Human PAD4 regulates histone arginine methylation levels via demethylimination." in: Science (New York, N.Y.), Vol. 306, Issue 5694, pp. 279-83, 2004 (PubMed).
|
Alternatives for antigen "Peptidyl Arginine Deiminase, Type IV (PADI4)", type "Antibodies"
|
Hosts
|
Rabbit (38), Mouse (8), Goat (4)
|
|
Reactivities
|
Human (48), Mouse (Murine) (7), Rat (Rattus) (5), Chicken (1), Chimpanzee (1), Cow (Bovine) (1), Dog (Canine) (1), Xenopus laevis (1), Zebrafish (1)
|
|
Applications
|
Western Blotting (WB) (33), Immunofluorescence (IF) (22), ELISA (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunohistochemistry (IHC) (7), Immunoprecipitation (IP) (7), Flow Cytometry (FACS) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
|
|
Conjugates
|
Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
|
|
Epitopes
|
C-Term (25), N-Term (6), AA 299-588 (5), AA 200-250,Internal Region (1), Center (1), Internal Region (1)
|
