Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 400-450)

Details for Product anti-BECN1 Antibody No. ABIN349986, Supplier: Log in to see
Antigen
  • beclin-1
  • atg6
  • vps30
  • beclin1
  • Beclin-1
  • becn1
  • zgc:73097
  • ATG6
  • VPS30
  • 4921513J16Rik
  • 5430417M23Rik
  • Atg6
  • beclin 1, autophagy related
  • beclin 1
  • beclin 1 protein
  • beclin-1
  • Beclin-1
  • beclin 1 (coiled-coil, myosin-like BCL2 interacting protein)
  • beclin-1-like
  • BECN1
  • becn1
  • atg6
  • CpipJ_CPIJ011087
  • PTRG_07825
  • SJAG_01876
  • Becn1
  • LOC100352353
Alternatives
anti-Human Beclin 1 antibody for Immunofluorescence (fixed cells)
Epitope
AA 400-450
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25
20
16
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13
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10
9
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8
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3
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3
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2
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2
1
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1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
424
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143
34
23
19
18
14
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13
8
6
3
3
2
2
1
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1
Host
Sheep
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2
Clonality
Polyclonal
Conjugate
This Beclin 1 antibody is un-conjugated
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23
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Application
Immunohistochemistry (IHC), Western Blotting (WB)
338
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159
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102
72
60
26
26
23
13
6
3
2
1
1
Options
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Immunogen A synthetic peptide from aa region 400-450 of human Beclin-1 conjugated to blue carrier protein has been used as the antigen. The antigen is homologous in rat and mouse.
Isotype IgG
Specificity Specific for Beclin-1.
Alternative Name Beclin-1 (BECN1 Antibody Abstract)
Background Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly.autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels ofautophagy have been described in some malignant tumors, and a role forautophagy in controlling the unregulated cell growth linked to cancer has been proposed. It may play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. May play a role inautophagy.
Subcellular location: Cytoplasm. Golgi apparatus membrane, Peripheral membrane protein. Note: Expressed in dendrites and cell bodies of cerebellar Purkinje cells
Tissue specificity: Ubiquitous Also known as: Beclin-1, BECN1, GT197, ATG6, VPS30, beclin1.
Pathways Autophagy
Application Notes Use at a concentration of 10-50 µg/ml.
The optimal working concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Lee, Jeong, Yoo, Lee: "Mutational analysis of NOTCH1, 2, 3 and 4 genes in common solid cancers and acute leukemias." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 115, Issue 12, pp. 1357-63, 2008 (PubMed).

Koneri, Goi, Hirono, Katayama, Yamaguchi: "Beclin 1 gene inhibits tumor growth in colon cancer cell lines." in: Anticancer research, Vol. 27, Issue 3B, pp. 1453-7, 2007 (PubMed).

Kihara, Kabeya, Ohsumi, Yoshimori: "Beclin-phosphatidylinositol 3-kinase complex functions at the trans-Golgi network." in: EMBO reports, Vol. 2, Issue 4, pp. 330-5, 2001 (PubMed).

Liang, Jackson, Seaman, Brown, Kempkes, Hibshoosh, Levine: "Induction of autophagy and inhibition of tumorigenesis by beclin 1." in: Nature, Vol. 402, Issue 6762, pp. 672-6, 2000 (PubMed).

Aita, Liang, Murty, Pincus, Yu, Cayanis, Kalachikov, Gilliam, Levine: "Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21." in: Genomics, Vol. 59, Issue 1, pp. 59-65, 1999 (PubMed).

Rommens, Durocher, McArthur, Tonin, LeBlanc, Allen, Samson, Ferri, Narod, Morgan: "Generation of a transcription map at the HSD17B locus centromeric to BRCA1 at 17q21." in: Genomics, Vol. 28, Issue 3, pp. 530-42, 1996 (PubMed).