Kir2.2 antibody (Cytoplasmic Domain)

Catalog No. ABIN350390

The Rabbit Polyclonal anti-Kir2.2 antibody has been validated for WB and IHC. It is suitable to detect Kir2.2 in samples from Rat.

Quick Overview for Kir2.2 antibody (Cytoplasmic Domain) (ABIN350390)

Target: Kir2.2 (KCNJ12) (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 12 (KCNJ12))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Kir2.2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Kir2.2 Antibody

Binding Specificity: Cytoplasmic Domain

Purpose: Rabbit antibody to KCNJ12

Specificity: Specific for KCNJ12.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from the cytoplasmic domain of rat KCNJ12 (KCNJN1 Kir2.2 IRK2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Kir2.2

Target: Kir2.2 (KCNJ12) (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 12 (KCNJ12))

Alternative Name: KCNJ12

Background: FUNCTION: Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium, as external potassium is raised the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Tissue specificity: Highest level in cerebellum. Moderately found in kidney forebrain and skeletal muscle. Not detected in uterus liver and pancreas. Subcellular location: Membrane Multi-pass membrane protein.

UniProt: P52188