Reticulon 4 antibody (AA 460-510)

Catalog No. ABIN350522

The Rabbit Polyclonal anti-Reticulon 4 antibody has been validated for WB and IHC. It is suitable to detect Reticulon 4 in samples from Human.

Quick Overview for Reticulon 4 antibody (AA 460-510) (ABIN350522)

Target: Reticulon 4 (RTN4)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Reticulon 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Reticulon 4 Antibody

Binding Specificity: AA 460-510

Purpose: Rabbit antibody to NOGO (460-510)

Specificity: Specific for Nogo.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 460-510 of human NOGO conjugated to an immunogenic carrier protein was used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for Reticulon 4

Target: Reticulon 4 (RTN4)

Alternative Name: NOGO

Background: Function: Potent neurite growth inhibitor in vitro and plays a role both in the restriction of axonal regeneration after injury and in structural plasticity in the CNS. Subcellular location: Endoplasmic reticulum membrane, Multi-pass membrane protein. Note: Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains. Tissue specificity: Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain skeletal muscle and adipocytes. Isoform 4 is testis-specific.

UniProt: Q9NQC3

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome