N-Ethylmaleimide-Sensitive Factor (NSF) (N-Term) antibody

Antigen: N-Ethylmaleimide-Sensitive Factor (NSF)

Synonyms: SKD2, NSF, MGC139541, nsf, MGC145265, 18.m06598, DKFZp459E222

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350528

Additional Information

Alternative name: NSF (Vesicle fusing ATPase)

UniProt: P46459

Immunogen: A synthetic peptide from the n-terminal of human NSF (Vesicle fusing ATPase) conjugated to an immunogenic carrier protein was used as the immunogen. The immunogen is homologous in mouse and rat.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. COFACTOR: Binds 1 magnesium ion per subunit. SUBUNIT: Homohexamer. Interacts with GABARAP and GABARAPL2. SUBCELLULAR LOCATION: Cytoplasm. Also known as: Vesicle fusing ATPase, N-ethylmaleimide sensitive fusion protein, NEM sensitive fusion protein, Vesicular fusion protein NSF.

Specificity: Appears to be specific for NSF.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Purification: Immunoaffinity chromatography

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Puerier, Coulter, Liang et al.: "Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in yeast." in: FEBS letters, Vol. 351, Issue 2, pp. 286-90, 1994 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Ballif, Carey, Sunyaev et al.: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).