N-Ethylmaleimide-Sensitive Factor (NSF) (N-Term) antibody

Details for Product No. ABIN350528
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Antigen
Synonyms NSF, 18.m06598, N-ethylmaleimide sensitive factor, T1J1.4, T1J1_4, AI316878, AU020090, AU067812, SKD2, nsf, si:bz18k17.1, wu:fj33g11
Epitope
N-Term
(16), (8), (3), (3), (2), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(39), (38), (37), (20), (13), (12), (4), (1), (1), (1)
Host
Rabbit
(51), (12)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(52), (16), (15), (10), (7), (6), (4), (3), (2), (2), (2), (1)
Pubmed 3 references available
Quantity 500 μg
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Catalog No. ABIN350528
454.67 $
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Immunogen A synthetic peptide from n-terminal of human NSF conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in mouse and rat.
Isotype IgG
Specificity Specific for NSF.
Alternative Name NSF
Background Function: Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.
Catalytic activity: ATP + H2O = ADP + phosphate.
Cofactor: Binds 1 magnesium ion per subunit.
Subunit: Homohexamer. Interacts with GABARAP and GABARAPL2.
Subcellular location: Cytoplasm. Also known as: Vesicle fusing ATPase, N-ethylmaleimide sensitive fusion protein, NEM sensitive fusion protein, Vesicular fusion protein NSF.
Research Area Signaling, Metabolism
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Puerier, Coulter, Liang et al.: "Identification of a novel mammalian member of the NSF/CDC48p/Pas1p/TBP-1 family through heterologous expression in yeast." in: FEBS letters, Vol. 351, Issue 2, pp. 286-90, 1994 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome. ..." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Ballif, Carey, Sunyaev et al.: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).

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