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Vacuolar Protein Sorting 35 Homolog (S. Cerevisiae) (vps35) (N-Term) antibody

Details for Product No. ABIN350530, Supplier: Login to see
Antigen
  • VPS35
  • 23.m05999
  • DKFZp459E1510
  • CG5625
  • DVps35
  • Dmel\\CG5625
  • Dvps35
  • dvps35
  • vps35
  • MGC81959
  • bZ34G2.3
  • si:rp71-34g2.3
  • wu:fa66e03
  • wu:fb95c08
  • zgc:136268
  • AI647796
  • Mem3
  • MEM3
  • PARK17
Epitope
N-Term
20
3
2
2
2
1
1
Reactivity
Mouse (Murine), Rat (Rattus), Human
33
11
8
5
1
1
1
1
1
Host
Rabbit
20
11
3
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
33
19
19
14
8
3
2
1
1
1
Supplier
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Immunogen A synthetic peptide from the n-terminal human Vacuolar protein sorting-associated protein 35 (Vesicle protein sorting 35, VPS35, MEM3) conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat, mouse.
Isotype IgG
Specificity Specific for VPS35.
Alternative Name VPS35 (vps35 Antibody Abstract)
Background Function: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
Subcellular location: Cytoplasm. Membrane, Peripheral membrane protein.
Tissue specificity: Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon. Also known as: Vesicle protein sorting 35, Maternal-embryonic 3, VPS35, MEM3.
Research Area Signaling
Pathways
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user. Not tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Hierro, Rojas, Rojas et al.: "Functional architecture of the retromer cargo-recognition complex." in: Nature, Vol. 449, Issue 7165, pp. 1063-7, 2007 (PubMed).

Vergues, Luton, Gruber et al.: "The mammalian retromer regulates transcytosis of the polymeric immunoglobulin receptor." in: Nature cell biology, Vol. 6, Issue 8, pp. 763-9, 2004 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Haft, de la Luz Sierra, Bafford et al.: "Human orthologs of yeast vacuolar protein sorting proteins Vps26, 29, and 35: assembly into multimeric complexes." in: Molecular biology of the cell, Vol. 11, Issue 12, pp. 4105-16, 2000 (PubMed).

Edgar, Polak: "Human homologues of yeast vacuolar protein sorting 29 and 35." in: Biochemical and biophysical research communications, Vol. 277, Issue 3, pp. 622-30, 2000 (PubMed).