SHANK3 antibody

Catalog No. ABIN350832

The Rabbit Polyclonal anti-SHANK3 antibody (ABIN350832) specifically detects SHANK3 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for SHANK3 antibody (ABIN350832)

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHANK3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SHANK3 Antibody

Purpose: Rabbit antibody to SHANK3

Specificity: Specific for SHANK3.

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from human SHANK3 conjugated to blue carrier protein was used as the antigen.

Isotype: IgG

Application Details

Application Notes: IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for SHANK3

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Alternative Name: SHANK3

Background: Function: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer respectively and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. Subcellular location: Cytoplasm. Cell junction ‰ synapse. Cell junction ‰ synapse ‰ postsynaptic cell membrane ‰ postsynaptic density. Note: Postsynaptic density of neuronal cells. Extends into the region subjacent to the PSD. Tissue specificity: Widely expressed in brain.

UniProt: Q9BYB0

Pathways: Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity