Synaptotagmin I (SYT1) antibody

Antigen: Synaptotagmin I (SYT1)

Synonyms: P65, SYT, SVP65, DKFZp781D2042, AW124717, G630098F17Rik, D. Syt I, DSYT, DSYT2, SYT I, SYT1, SytI, dsyt1, dsytI, l(2)23AB1, l(2)23Ba, l(2)k05909, syt-1, DmelCG3139, CG3139, Syti, GB20036, syt, svp65, DKFZp459D121, DKFZP459P193, p65

Clonality: Polyclonal

Host: Sheep

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN350918

Additional Information

Alternative name: Synaptotagmin-1 (Synaptotagmin I)

UniProt: P21579

Immunogen: A synthetic peptide from human Synaptotagmin-1 (Synaptotagmin I) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Note: Synaptic vesicles and chromaffin granules. TISSUE SPECIFICITY: Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues. Also known as: Synaptotagmin-1, Synaptotagmin I, SytI, p65, SVP65.

Specificity: Appears to be specific for Synaptotagmin-1.

Synonyms: P65, SYT, SVP65, DKFZp781D2042, AW124717, G630098F17Rik, D. Syt I, DSYT, DSYT2, SYT I, SYT1, SytI, dsyt1, dsytI, l(2)23AB1, l(2)23Ba, l(2)k05909, syt-1, DmelCG3139, CG3139, Syti, GB20036, syt, svp65, DKFZp459D121, DKFZP459P193, p65

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Perin, Fried, Mignery et al.: "Phospholipid binding by a synaptic vesicle protein homologous to the regulatory region of protein kinase C." in: Nature, Vol. 345, Issue 6272, pp. 260-3, 1990 (PubMed).