GPC4 antibody (Internal Region)

Catalog No. ABIN351425

Product Details anti-GPC4 Antibody

Target: GPC4 (Glypican 4 (GPC4))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Hamster, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPC4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GPC4 / Glypican 4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Bat, Hamster (100%) Marmoset, Dog, Elephant, Panda, Rabbit (94%) Bovine, Horse, Pig (88%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from internal region of human GPC4 / Glypican 4. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Bat, Hamster (100%), Marmoset, Dog, Elephant, Panda, Rabbit (94%), Bovine, Horse, Pig (88%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GPC4

Target: GPC4 (Glypican 4 (GPC4))

Alternative Name: GPC4 / Glypican 4 (GPC4 Products)

Synonyms: K-glypican antibody, knypek antibody, xgly4 antibody, Xgly4 antibody, glypican-4 antibody, 9530073D23Rik antibody, AF311610 antibody, AI385680 antibody, AI661372 antibody, chunp6920 antibody, gpc4, antibody, gpc4/6 antibody, gpc6 antibody, kny antibody, wu:fc47a08 antibody, wu:fe05f10 antibody, glypican 4 antibody, glypican 4 L homeolog antibody, GPC4 antibody, gpc4.L antibody, gpc4 antibody, Gpc4 antibody

Background: Name/Gene ID: GPC4

Synonyms: GPC4, DJ900E8.1 (glypican 4), Glypican 4, Glypican proteoglycan 4, Glypican-4, K-glypican

Gene ID: 2239

Pathways: Glycosaminoglycan Metabolic Process