ARPC2 antibody (AA 288-300)

Catalog No. ABIN351464

The Goat Polyclonal anti-ARPC2 antibody is suitable to detect ARPC2 in samples from Human, Mouse, Rat, Cow, Rabbit, Dog, Hamster and Monkey. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for ARPC2 antibody (AA 288-300) (ABIN351464)

Target: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Hamster, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This ARPC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ARPC2 Antibody

Binding Specificity: AA 288-300

Specificity: Human ARPC2. The reported variants NP_005722.1 and NP_690601.1 represent identical protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Opossum, Platypus (100%) Horse, Lizard (92%) Turkey, Chicken, Xenopus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EMKTITGKTFSSR from the C-terminus of human ARPC2 (NP_005722.1, NP_690601.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Opossum, Platypus (100%), Horse, Lizard (92%), Turkey, Chicken, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (0.1 - 0.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ARPC2

Target: ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))

Alternative Name: ARPC2 / p34-Arc

Background: Name/Gene ID: ARPC2
Family: Cytoskeleton

Synonyms: ARPC2, Arp2/3 complex 34 kDa subunit, p34-Arc, PNAS-139, PRO2446, ARC34

Gene ID: 10109

NCBI Accession: NP_005722, NP_690601

UniProt: O15144

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization