AKR1A1 antibody (AA 313-325)

Catalog No. ABIN351467

The Goat Polyclonal anti-AKR1A1 antibody is suitable to detect AKR1A1 in samples from Human, Mouse, Rat, Pig, Cow, Dog, Horse, Rabbit, Monkey, Bat, Hamster and Xenopus laevis. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for AKR1A1 antibody (AA 313-325) (ABIN351467)

Target: AKR1A1 (Aldo-Keto Reductase Family 1, Member A1 (Aldehyde Reductase) (AKR1A1))

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Horse, Rabbit, Monkey, Bat, Hamster, Xenopus laevis

Host: Goat

Clonality: Polyclonal

Conjugate: This AKR1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-AKR1A1 Antibody

Binding Specificity: AA 313-325

Specificity: Human AKR1A1. Both reported variants (NP_006057.1and NP_697021.1) represent identical protein

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Pig, Xenopus (100%) Turkey, Chicken, Platypus, Catfish, Zebrafish (92%) Opossum, Salmon, Stickleback, Pike (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DAGHPLYPFNDPY from the C-terminus of human AKR1A1 (NP_006057.1, NP_697021.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Pig, Xenopus (100%), Turkey, Chicken, Platypus, Catfish, Zebrafish (92%), Opossum, Salmon, Stickleback, Pike (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for AKR1A1

Target: AKR1A1 (Aldo-Keto Reductase Family 1, Member A1 (Aldehyde Reductase) (AKR1A1))

Alternative Name: AKR1A1

Background: Name/Gene ID: AKR1A1

Synonyms: AKR1A1, ALDR1, Aldehyde reductase, Alcohol dehydrogenase, ARM, DD3, Dihydrodiol dehydrogenase 3, ALR

Gene ID: 10327

NCBI Accession: NP_006057, NP_697021

UniProt: P14550

Pathways: Monocarboxylic Acid Catabolic Process