COPS2 antibody (AA 439-451)

Catalog No. ABIN351473

The Goat Polyclonal anti-COPS2 antibody is suitable to detect COPS2 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Monkey, Pig, Chicken, Xenopus laevis and Bat. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for COPS2 antibody (AA 439-451) (ABIN351473)

Target: COPS2 (COP9 Constitutive Photomorphogenic Homolog Subunit 2 (Arabidopsis) (COPS2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Monkey, Pig, Chicken, Xenopus laevis, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This COPS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-COPS2 Antibody

Binding Specificity: AA 439-451

Specificity: Human COPS2.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Xenopus (100%) Zebrafish (92%) Stickleback (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-LNSLNQAVVSKLA from the C-terminus of human COPS2 (NP_004227.1, NP_001137359.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Xenopus (100%), Zebrafish (92%), Stickleback (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:16000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for COPS2

Target: COPS2 (COP9 Constitutive Photomorphogenic Homolog Subunit 2 (Arabidopsis) (COPS2))

Alternative Name: COPS2 / TRIP15 / ALIEN

Background: Name/Gene ID: COPS2

Synonyms: COPS2, Alien homolog, ALIEN, SGN2, TRIP15, TR-interacting protein 15, Signalosome subunit 2, TRIP-15

Gene ID: 9318

NCBI Accession: NP_004227, NP_001137359

UniProt: P61201

Pathways: Cell Division Cycle