ACTR1B antibody (AA 363-375)

Catalog No. ABIN351475

This Goat Polyclonal antibody specifically detects ACTR1B in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Monkey and Pig.

Quick Overview for ACTR1B antibody (AA 363-375) (ABIN351475)

Target: ACTR1B (ARP1 Actin-Related Protein 1 Homolog B, Centractin beta (ACTR1B))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Monkey, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This ACTR1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ACTR1B Antibody

Binding Specificity: AA 363-375

Specificity: Human ACTR1B. This epitope was selected to minimise the chance of cross-reactivity with ACTR1A (YEEDGARSIHRKT).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Monkey, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Horse, Rabbit, Pig (100%) Hamster, Bat, Opossum, Turkey, Chicken, Platypus, Xenopus (92%) Gorilla, Gibbon, Elephant, Salmon, Stickleback, Zebrafish, Mosquito (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-YEEDGSRAIHRKT from the C-terminus of human ACTR1B (NP_005726.1). Percent identity by BLAST analysis: Human, Monkey, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Horse, Rabbit, Pig (100%), Hamster, Bat, Opossum, Turkey, Chicken, Platypus, Xenopus (92%), Gorilla, Gibbon, Elephant, Salmon, Stickleback, Zebrafish, Mosquito (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:128000), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ACTR1B

Target: ACTR1B (ARP1 Actin-Related Protein 1 Homolog B, Centractin beta (ACTR1B))

Alternative Name: ACTR1B / PC3

Background: Name/Gene ID: ACTR1B

Synonyms: ACTR1B, Actin-related protein 1B, Beta-centractin, Centractin beta, PC3, ARP1B, CTRN2

Gene ID: 10120

NCBI Accession: NP_005726

UniProt: P42025