Ubiquitin-Activating Enzyme 3 (UBA3) (C-Term) antibody

Antigen: Ubiquitin-Activating Enzyme 3 (UBA3)

Synonyms: UBE1C, hUBA3, MGC22384, DKFZp566J164, Ube1c, ube1c, zgc:55528, wu:fb75e04, wu:fc37b11, MGC140052, DKFZp459A0915, DKFZp459D1713, DKFZp459G1962, AI256736, AI848246, AW546539, A830034N06Rik

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN356593

Additional Information

Characteristics: Synonyms: Ubiquitin-activating enzyme 3, Ubiquitin-activating enzyme E1C, NEDD8-activating enzymeE1C, NEDD8-activating enzyme E1 catalytic subunit, Ubiquitin-like modifier-activatingenzyme 3

Alternative name: UBA3 / UBE1C

Gene ID: 9039

Swiss-Prot: Q8TBC4

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated syntheticpeptide selected from the C-terminal region of human UBE1C.

Cross-Reactivity: Human, Mouse (Murine)

Isotype: Ig  (Matching secondary antibodies)

Description: The modification of proteins with ubiquitin is an important cellular mechanism fortargeting abnormal or short-lived proteins for degradation. Ubiquitination involves at leastthree classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugatingenzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of theE1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, anamyloid beta precursor protein binding protein, to form a heterodimer, and then theenzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division,signaling and embryogenesis. Multiple alternatively spliced transcript variants encodingdistinct isoforms have been found for this gene.

Specificity: This antibody is specific to UBE1C (C-term). Predicted to cross react with Mouse and DANRE (100% Antigen Homology). Species: Human and Mouse. Others not tested.

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/100~1/500. Immunohistochemistry: 1/50~1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: PBS containing 0.09% (W/V) Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Restrictions: For Research Use only