SLC29A1 antibody (Middle Region)

Catalog No. ABIN356634

Cited in 1 publication. The Rabbit Polyclonal anti-SLC29A1 antibody (ABIN356634) specifically detects SLC29A1 in WB, IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for SLC29A1 antibody (Middle Region) (ABIN356634)

Target: SLC29A1 (Solute Carrier Family 29 (Nucleoside Transporters), Member 1 (SLC29A1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC29A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SLC29A1 Antibody

Binding Specificity: Middle Region

Specificity: This antibody is specific to ENT1 (Center).

Purification: Protein A Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human ENT1 (Slc29a1).

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1-50-1/100. Immunohistochemistry: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-SLC29A1 antibody (ABIN356634)

Wang, Word, Lyn-Cook: "Enhanced efficacy of gemcitabine by indole-3-carbinol in pancreatic cell lines: the role of human equilibrative nucleoside transporter 1." in: Anticancer research, Vol. 31, Issue 10, pp. 3171-80, (2011) (PubMed).

Target Details for SLC29A1

Target: SLC29A1 (Solute Carrier Family 29 (Nucleoside Transporters), Member 1 (SLC29A1))

Alternative Name: SLC29A1 / ENT1

Background: ENT1 is a member of the equilibrative nucleoside transporter family. It is a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies.Synonyms: Equilibrative nucleoside transporter 1, Solute carrier family 29 member 1

Gene ID: 2030, 9606

UniProt: Q99808

Pathways: Carbohydrate Homeostasis, Synaptic Membrane