LIN28B antibody (Lin-28 Homolog B (C. Elegans)) (Center)

Details for Product anti-LIN28B Antibody No. ABIN357013, Supplier: Log in to see
Antigen
  • csdd2
  • LIN-28
  • CSDD2
  • 2810403D23Rik
  • D030047M17Rik
  • Lin-28.2
  • lin-28 homolog B (C. elegans)
  • lin-28 homolog B
  • LIN28B
  • lin28b
  • LOC100221494
  • Lin28b
Epitope
Center
17
14
9
6
5
5
4
3
2
1
1
1
1
1
Reactivity
Human
77
17
15
1
1
1
1
1
Host
Rabbit
77
2
Clonality
Polyclonal
Conjugate
This LIN28B antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
56
34
32
28
26
14
10
7
4
2
2
Options
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Immunogen KLH conjugated synthetic peptide selected from the Center region of human LIN28B
Isotype Ig
Specificity This antibody detects LIN28B at center.
Cross-Reactivity (Details) Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name LIN28B (LIN28B Antibody Abstract)
Background Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.Synonyms: CSDD2, LIN28B, Lin-28 homolog B
Molecular Weight 26953 Da
Gene ID 389421, 9606
UniProt Q6ZN17
Application Notes ELISA 1: 1,000. Western blot 1: 50 - 1: 100. Immunohistochemistry 1: 10 - 1: 50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % (W/V) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-LIN28B antibody (Lin-28 Homolog B (C. Elegans)) (Center) (ABIN357013) anti-Lin-28 Homolog B (C. Elegans) (LIN28B) (Center) antibody
 image for anti-LIN28B antibody (Lin-28 Homolog B (C. Elegans)) (Center) (ABIN357013) anti-Lin-28 Homolog B (C. Elegans) (LIN28B) (Center) antibody (Image 2)
Background publications Guo, Chen, Ito, Watanabe, Ge, Kodama, Aburatani: "Identification and characterization of lin-28 homolog B (LIN28B) in human hepatocellular carcinoma." in: Gene, Vol. 384, pp. 51-61, 2006 (PubMed).