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DKK4 antibody (Dickkopf Homolog 4 (Xenopus Laevis)) (AA 7-36)

Details for Product anti-DKK4 Antibody No. ABIN357073, Supplier: Log in to see
Antigen
  • Dkk-4
  • DKK-4
  • RGD1563172
Alternatives
anti-Human DKK4 antibody for Western Blotting
Epitope
AA 7-36, N-Term
52
21
16
5
3
2
2
2
2
1
1
Reactivity
Human
90
16
5
2
1
1
1
1
Host
Rabbit
83
15
2
Clonality
Polyclonal
Conjugate
This DKK4 antibody is un-conjugated
8
8
7
7
7
7
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
86
60
28
9
2
2
2
Supplier
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Immunogen KLH conjugated synthetic peptide between 7~36 amino acids from the N-terminal region of Human DKK4
Sequence DKK4
Isotype Ig
Specificity This antibody detects DKK4 at N-term.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name Dickkopf-4 (DKK4) (DKK4 Antibody Abstract)
Background DKK4, like DKK1, DKK2, and DKK3, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T-cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.Synonyms: Dickkopf-related protein 4, Dkk-4
Molecular Weight 24875 Da
Gene ID 27121, 9606
UniProt Q9UBT3
Pathways WNT Signaling
Application Notes ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-DKK4 antibody (Dickkopf Homolog 4 (Xenopus Laevis)) (AA 7-36) (ABIN357073) anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (AA 7-36), (N-Term) antibody
 image for anti-DKK4 antibody (Dickkopf Homolog 4 (Xenopus Laevis)) (AA 7-36) (ABIN357073) anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (AA 7-36), (N-Term) antibody (Image 2)
 image for anti-DKK4 antibody (Dickkopf Homolog 4 (Xenopus Laevis)) (AA 7-36) (ABIN357073) anti-Dickkopf Homolog 4 (Xenopus Laevis) (DKK4) (AA 7-36), (N-Term) antibody (Image 3)
Background publications Yoshida, Satoh, Mitsuya et al.: "Assignment of the human dickkopf (Xenopus) homolog 4 (DKK4) to chromosome 8p11.2-->p11.1 by fluorescence in situ hybridization." in: Cytogenetics and cell genetics, Vol. 94, Issue 1-2, pp. 88-9, 2001 (PubMed).

Krupnik, Sharp, Jiang et al.: "Functional and structural diversity of the human Dickkopf gene family." in: Gene, Vol. 238, Issue 2, pp. 301-13, 1999 (PubMed).