SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (N-Term) antibody

Details for Product No. ABIN357471
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Antigen
Synonyms SMURF2, 2810411E22Rik, AI558114, AI649275, RGD1310067, wu:fb82a11, wu:fc41c02
Epitope
N-Term
(13), (8), (6), (2), (2), (1), (1)
Reactivity
Human, Mouse (Murine)
(41), (17), (13), (12), (12)
Host
Rabbit
(40), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(32), (14), (14), (10), (9), (8), (2), (1), (1)
Pubmed 3 references available
Quantity 0.4 mL
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Catalog No. ABIN357471
357.50 $
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SMURF2.
Isotype Ig
Specificity This antibody detects SMURF2 at N-term.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Alternative Name SMURF2
Background SMURF2, a 748-amino acid ubiquitin E3 ligase that is 83 % identical to SMURF1, codes for a C2-WW-HECT domain ubiquitin ligase that associates constitutively with SMAD7. Binding to SMAD7 induces export of SMURF2 and recruitment to the activated transforming growth factor-beta receptor (TGFBR), where it causes receptor and SMAD7 degradation. A strong interaction of second and third SMURF2 WW domains has been identified with SMAD1, SMAD2, and SMAD3, but not SMAD4. Western blot analysis showed that SMURF2 selectively downregulates the transcription of SMAD2 and SMAD1, but not SMAD3. The nuclear SMURF2/phosphorylated SMAD2 interaction is requires TGFB1.Synonyms: E3 ubiquitin-protein ligase SMURF2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMURF 2, SMURF-2
Molecular Weight 86195 Da
Gene ID 64750
NCBI Accession NP_073576
UniProt Q9HAU4
Application Notes ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (N-Term) antibody anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (N-Term) antibody
anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (N-Term) antibody (2) anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (N-Term) antibody (Image 2)
Background publications Lin, Liang, Feng: "Smurf2 is a ubiquitin E3 ligase mediating proteasome-dependent degradation of Smad2 in transforming growth factor-beta signaling." in: The Journal of biological chemistry, Vol. 275, Issue 47, pp. 36818-22, 2001 (PubMed).

Zhang, Chang, Gehling et al.: "Regulation of Smad degradation and activity by Smurf2, an E3 ubiquitin ligase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 3, pp. 974-9, 2001 (PubMed).

Kavsak, Rasmussen, Causing et al.: "Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF beta receptor for degradation." in: Molecular cell, Vol. 6, Issue 6, pp. 1365-75, 2001 (PubMed).

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