LEO1 antibody (pSer10)

Catalog No. ABIN358507

This Rabbit Polyclonal antibody specifically detects LEO1 in EIA. It exhibits reactivity toward Human.

Quick Overview for LEO1 antibody (pSer10) (ABIN358507)

Target: LEO1 (Leo1, Paf1/RNA Polymerase II Complex Component, Homolog (S. Cerevisiae) (LEO1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LEO1 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-LEO1 Antibody

Binding Specificity: pSer10

Specificity: This antibody detects LEO1 pSer10.

Purification: Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human LEO1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for LEO1

Target: LEO1 (Leo1, Paf1/RNA Polymerase II Complex Component, Homolog (S. Cerevisiae) (LEO1))

Alternative Name: LEO1

Background: The PAF1 complex is a multifunctional complex. The PAF1 complex interacts with POLR2A. May be involved in both initiation and elongation, histone methylation and RNA processing. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts.Synonyms: LEO-1, RDL, RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein

Molecular Weight: 75273 Da

Gene ID: 123169, 9606

UniProt: Q8WVC0

Pathways: Stem Cell Maintenance