LEO1 antibody (C-Term)

Catalog No. ABIN6242423

Product Details anti-LEO1 Antibody

Target: LEO1 (Leo1, Paf1/RNA Polymerase II Complex Component, Homolog (S. Cerevisiae) (LEO1))

Binding Specificity: AA 616-650, C-Term

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LEO1 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Mouse Leo1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 616-650 amino acids from the C-terminal region of Mouse Leo1.

Clone: RB50947

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for LEO1

Target: LEO1 (Leo1, Paf1/RNA Polymerase II Complex Component, Homolog (S. Cerevisiae) (LEO1))

Alternative Name: Leo1 (LEO1 Products)

Synonyms: LEO1 antibody, RDL antibody, Gm185 antibody, zgc:77374 antibody, LEO1 homolog, Paf1/RNA polymerase II complex component antibody, Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) antibody, RNA polymerase-associated protein LEO1 antibody, LEO1 homolog, Paf1/RNA polymerase II complex component S homeolog antibody, Leo1, Paf1/RNA polymerase II complex component antibody, LEO1 antibody, leo1 antibody, LOC100466503 antibody, leo1.S antibody, Leo1 antibody, LOC415420 antibody, LOC478310 antibody

Background: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys- 4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling (By similarity).

Molecular Weight: 75597

UniProt: Q5XJE5

Pathways: Stem Cell Maintenance