DAP Kinase 1 antibody (C-Term)

Catalog No. ABIN359411

The Rabbit Polyclonal anti-DAP Kinase 1 antibody is suitable to detect DAP Kinase 1 in samples from Human and Mouse. It has been validated for WB, IHC (p) and EIA.

Quick Overview for DAP Kinase 1 antibody (C-Term) (ABIN359411)

Target: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAP Kinase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-DAP Kinase 1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects DAP Kinase 1 at C-term.

Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DAPK1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/100-1/500Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month (in aliquots) at-20 °C for longer.

Target Details for DAP Kinase 1

Target: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Alternative Name: DAPK1

Background: Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death (apoptosis). DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate.Synonyms: DAP kinase 1, DAPK, DAPK-1, DKFZp781I035, Death-associated protein kinase 1

Molecular Weight: 160220 Da

Gene ID: 1612, 9606

UniProt: P53355

Pathways: MAPK Signaling, Interferon-gamma Pathway