Angiopoietin 4 antibody

Catalog No. ABIN360871

This Rat Monoclonal antibody specifically detects Angiopoietin 4 in WB. It exhibits reactivity toward Mouse.

Quick Overview for Angiopoietin 4 antibody (ABIN360871)

Target: Angiopoietin 4 (ANGPT4)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Angiopoietin 4 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 2H34

Product Details anti-Angiopoietin 4 Antibody

Specificity: This antibody detects Mouse Angiopoietin-3 in Western blotting. Other species not tested.

Purification: Protein A/G affinity chromatography

Immunogen: Recombinant Angiopoietin-3

Isotype: IgG2

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL.

Buffer: 0.2 μm filtered PBS solution

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Angiopoietin 4

Target: Angiopoietin 4 (ANGPT4)

Alternative Name: Angiopoietin-4

Background: Mouse Angiopoietin3 (ANG3), is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structural motifs of angiopoietins including the coiled coiled domain near the amino-terminus and a fibrinogen like domain at the C-terminus. Mouse ANG 3 cDNA encodes a 509 amino acid (aa) precursor protein with a 21 aa signal peptide. It shares 47 % , 46 % and 54 % aa sequence identity with mouse ANG1, mouse ANG2 and human ANG4, respectively. Although the sequence homology is much higher between the human and mouse counterparts for ANG1 (97 %) and ANG2 (85 %), mouse ANG3 is believed to be an ortholog of human ANG4 based on chromosomal localization studies. Human ANG4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse ANG3 is expressed in multiple mouse tissues. Human ANG4 is an agonist that can bind and activate Tie2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Mouse ANG3 has been reported to be a Tie2 antagonist. It is likely that mouse ANG3, like ANG2, may exert agonist or antagonist activities depending on the cell context.Synonyms: ANG-3, ANG-4, ANG3, ANG4, ANGPT4

Gene ID: 11602

NCBI Accession: NP_033771

UniProt: Q9WVH6

Pathways: RTK Signaling