ARTN antibody

Catalog No. ABIN360873

Product Details anti-ARTN Antibody

Target: ARTN (Artemin (ARTN))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ARTN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody detects Mouse Artemin.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on Protein A/G.

Immunogen: Mouse Recombinant Artemin.

Clone: 6G32

Isotype: IgG2

Application Details

Application Notes: Western Blot: 1/500-1/1000. Immunohistochemistry on Frozen Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 200 μL sterile PBS (final concentration = 500 μg/mL).

Buffer: 0.2 μm filtered PBS solution

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ARTN

Target: ARTN (Artemin (ARTN))

Alternative Name: Artemin (ARTN Products)

Synonyms: ARTN antibody, ENOVIN antibody, EVN antibody, NBN antibody, neublastin antibody, artemin antibody, ARTN antibody, Artn antibody

Background: Artemin is a member of the Glia Cel-lDerived Neurotrophic factor (GDNF) family ligands, which include GDNF, Persephin, Artemin, and Neurturin. GDNF family ligands are distant members of the Transforming Growth Factor β (TGFβ) superfamily. Similar to other TGFβ family proteins, Artemin is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxyterminal domain. The carboxy terminal domain of Artemin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active Artemin is a disulfide-linked homodimer of the carboxyterminal 113 amino acid residues. Mature mouse Artemin shares 88.5 % amino acid sequence similarity with human Artemin. Mature Artemin also shares approximately 40 % amino acid sequence identity with the other three members of the GDNF family ligands. Bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFRα1GFRα4) and a common signaling component, cRET (receptor tyrosine kinase). Artemin prefers to bind to GFRα3 and activites the GFRα3RET. However, in the presence of RET, it can bind to GFRα1 as well. Artemin has been shown to promote the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs.Synonyms: ARTN, EVN, Enovin, Neublastin

Molecular Weight: 24 kDa.

Gene ID: 11876

NCBI Accession: NP_033841

UniProt: Q9Z0L2