Intraflagellar Transport Protein 57 Homolog (IFT57) antibody

Antigen: Intraflagellar Transport Protein 57 Homolog (IFT57)

Synonyms: HIPPI, MHS4R2, ESRRBL1, FLJ10147, Esrrbl1, IFT57, MGC179556, hippi, mhs4r2, esrrbl1, Hippi, 4833420A15Rik, zgc:111831

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN361194

Additional Information

Alternative name: HIPPI

Gene ID: 55081, 73916 (mouse)

Immunogen: Peptide with sequence C-EQPQEYDDPNATISN, from the internal region of the protein sequence according to NP_060480.1

Description: All names and symbols: IFT57, intraflagellar transport 57 homolog (Chlamydomonas), ESRRBL1, FLJ10147, HIPPI, MHS4R2, HIP1 protein interactor, estrogen-related receptor beta like 1, huntingtin interacting protein-1 interacting protein. Official Symbol: IFT57. RefSeq number(s): NP_060480.1.

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Cow, Internal Region

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 49kDa band observed in Human and Mouse Brain lysates (calculated MW of 49.1kDa according to Human NP_060480.1 and of 48.8kDa according to Mouse NP_082956.2). Recommended concentration: 1-3µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Neurology

Restrictions: For Research Use only

Images

EB09292 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.