IFT57 antibody (Internal Region)

Catalog No. ABIN6262481

This Rabbit Polyclonal antibody specifically detects IFT57 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for IFT57 antibody (Internal Region) (ABIN6262481)

Target: IFT57 (Intraflagellar Transport 57 Homolog (IFT57))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFT57 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-IFT57 Antibody

Binding Specificity: Internal Region

Specificity: IFT57 Antibody detects endogenous levels of total IFT57.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human IFT57, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for IFT57

Target: IFT57 (Intraflagellar Transport 57 Homolog (IFT57))

Alternative Name: IFT57

Background:

Description: Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator, however the relevance of such function remains unclear.

Gene: IFT57

Molecular Weight: 49 kDa

Gene ID: 55081

UniProt: Q9NWB7

Pathways: Hedgehog Signaling, Positive Regulation of Endopeptidase Activity