Microtubule Associated Protein 2 (MAP2) antibody

Antigen: Microtubule Associated Protein 2 (MAP2)

Synonyms: MAP2A, MAP2B, MAP2C, DKFZp686I2148, MAP2, MAP-2, Mtap-2, xmap2, MGC53131, a730034c02, map2, MGC145864

Clonality: Polyclonal

Host: Chicken

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN361345

Additional Information

Description: Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein ( (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein ( (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006). Anti-MAP2 Left: Western blot of rat cortex lysate showing specific immunolabeling of the ~280k MAP2 protein. Right:Mixed neuron/glial cultures. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.

Specificity: Specific for the ~ 280k MAP2 protein.

Sensitivity: The antibody has been directly tested for reactivity in bovine, human, mouse and rat. It is expected that the antibody will react with other mammalian tissues.

Application Details

Application Notes: Recommended Dilution: WB: 1:20,000 IF: 1:2,500 Quality Control: Western blots performed on each lot.

Purification: Total IgY fraction

Buffer: Liquid containing 10mM sodium azide.

Storage: Store at -20 °C, stable for 1 year

Research Area: Cytoskeleton

Restrictions: For Research Use only

Images

Western blots of rat cortex lysate showing specific immunolabeling of the ~280k MAP2 protein.

Mixed neuron/glial cultures. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.