Kv2.2 Voltage-Gated Potassium Channel antibody

Antigen: Kv2.2 Voltage-Gated Potassium Channel

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN361380

Additional Information

Alternative name: Voltage Gated Potassium Channel, Kv2.2 Subunit

Format: Affinity-purified

Description: Voltage-gated K+ channels are important determinants of neuronal membrane excitability (Pongs, 1999). Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK)channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety of non-neuronal cells types including pancreatic islets, alveolar cells and cardiac myocytes (Hwang et al., 1993, Yan et al., 2004, Michaelevski et al., 2003). Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells (Blaine and Ribera, 1998, Burger and Ribera, 1996). Anti-Voltage Gated Potassium Channel, Kv2.2-Subunit Western blot of rat brain homogenate showing specific immunolabeling of the ~125k voltage-gated potassium channel, Kv2.2 (Control). The immunolabeling is blocked by preadsorption with the peptide used as antigen (Peptide Block).

Specificity: Specific for the ~125k voltage gated potassium channel, Kv 2.2 subunit.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC (frozen sections, unpublished observations): 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification using a Sulfo-Link® column matrix to which the peptide immunogen was coupled.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of rat brain homogenate showing specific immunolabeling of the ~125k voltage-gated potassium channel, Kv2.2 (Control). The immunolabeling is blocked by preadsorption with the peptide used as antigen (Peptide Block).