NMDA Receptor, NR2A Subunit (0) (N-Term) antibody

Antigen: NMDA Receptor, NR2A Subunit (0)

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN361397

Additional Information

Alternative name: NMDA Receptor, NR2A Subunit N-terminus

Format: Affinity-purified

Description: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Anti-NMDA Receptor, NR2A Subunit Western blot of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDA receptor.

Specificity: Specific for the ~180k NR2A subunit of the NMDA receptor.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC: 1:500 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDA receptor.