NMDA Receptor, NR1 Subunit antibody

Antigen: NMDA Receptor, NR1 Subunit

Clonality: Monoclonal (R1JHL)

Host: Mouse

Reactivity: Rat (Rattus), Mouse (Murine)

Application: Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN361405

Additional Information

Immunogen: Fusion protein containing amino acids 1-564 of the NR1 subunit of rat NMDA receptor.

Format: Lyophilized

Isotype: TBD

Clone: R1JHL

Description: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1-subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Anti-NMDAR, NR1 Subunit Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor.

Specificity: Specific for the ~120k NR1 subunit of the NMDA receptor. The antibody has been directly tested for reactivity in Western blots in rat and mouse tissues.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IP: 3 µg per 200 µg lysate

Purification: Culture supernatant

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor.

Publications

Publications: Zhang, Dong, Zhang et al.: "Isoflurane-induced caspase-3 activation is dependent on cytosolic calcium and can be attenuated by memantine." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 17, pp. 4551-60, 2008 (PubMed).

Davies, Goebel-Goody, Coultrap et al.: "Long term synaptic depression that is associated with GluR1 dephosphorylation but not alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33138-46, 2008 (PubMed).