GRIN1/NMDAR1 antibody (Internal Region)

Catalog No. ABIN185678

The Goat Polyclonal anti-GRIN1/NMDAR1 antibody is suitable to detect GRIN1/NMDAR1 in samples from Human and Mouse. It has been validated for ELISA and IF.

Quick Overview for GRIN1/NMDAR1 antibody (Internal Region) (ABIN185678)

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This GRIN1/NMDAR1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-GRIN1/NMDAR1 Antibody

Binding Specificity: Internal Region

Purpose: NMDA receptor 1 / GRIN1

Sequence: TQERVNNSNK KE

Specificity: This antibody is expected to recognise all three reported isoforms (NP_000823.4, NP_067544.1, NP_015566.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TQERVNNSNKKE, from the internal region of the protein sequence according to NP_000823.4, NP_067544.1, NP_015566.1.

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membrane and cytoplasm of MCF7 and NIH3T3 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GRIN1/NMDAR1

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Alternative Name: GRIN1

Background: GRIN1, NMDA receptor 1, glutamate receptor, ionotropic, N-methyl D-aspartate 1 , NMDA1, NMDAR1, NR1 , N-methyl-D-aspartate receptor channel, subunit zeta-1, glutamate [NMDA] receptor subunit zeta 1

Gene ID: 2902, 14810, 24408

NCBI Accession: NP_000823, NP_067544, NP_015566

Pathways: Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity