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Antibody
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anti-Choline O-Acetyltransferase CHAT
Choline O-Acetyltransferase (CHAT) antibody
| Antigen |
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| Synonyms |
CMS1A, CMS1A2, B230380D24Rik, CT23399, Cat, ChAT, cha, dChAT, l(3)91Cc, DmelCG12345, CG12345, CHAT, ChoAcTase, Ci-ChAT |
| Clonality |
Polyclonal |
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Host
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Reactivity
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 Alternatives Chicken, Guinea Pig, Human, Mouse (Murine), Primates, Rat (Rattus)
Human (44), Rat (Rattus) (38), Mouse (Murine) (33), Pig (Porcine) (19), Dog (Canine) (16), Guinea Pig (10), Rabbit (4), Chicken (3), Bird (Avian) (2), Monkey (2), Bat (1), Cat (Feline) (1), Primates (1)
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Application
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Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
Western Blotting (WB) (45), Immunohistochemistry (IHC) (28), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Immunofluorescence (IF) (15), Flow Cytometry (FACS) (13), ELISA (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunocytochemistry (ICC) (6), Enzyme Immunoassay (EIA) (5), Immunoprecipitation (IP) (3), Immunoelectron Microscopy (EM) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (1)
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| Catalog no. |
ABIN361419 |
| Quantity |
100 µl (Variants) |
| Price |
345.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business days |
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Alternative name
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Choline Acetyltransferase
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Format
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Affinity-purified
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Description
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Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999). Anti-Choline Acetyltransferase Western blot of rat brain lysate showing specific immunolabeling of the ~70k ChAT
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Specificity
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Specific for the ~ 70k choline acetyltransferase protein
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Application Notes
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Recommended Dilution: WB: 1:100 IHC: 1:100 Quality Control: Western blots performed on each lot.
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Purification
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Prepared from goat serum by affinity purification using a column to which the protein immunogen was coupled.
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Storage
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Store at -20 °C, stable for 1 year
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Research Area
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Neurology
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Restrictions
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For Research Use only
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Alternatives for antigen "Choline O-Acetyltransferase (CHAT)", type "Antibodies"
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Hosts
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Rabbit (41), Goat (16), Sheep (8), Mouse (7), Chicken (6)
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Reactivities
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Human (44), Rat (Rattus) (38), Mouse (Murine) (33), Pig (Porcine) (19), Dog (Canine) (16), Guinea Pig (10), Rabbit (4), Chicken (3), Bird (Avian) (2), Monkey (2), Bat (1), Cat (Feline) (1), Primates (1)
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Applications
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Western Blotting (WB) (45), Immunohistochemistry (IHC) (28), Immunohistochemistry (Frozen Sections) (IHC (fro)) (16), Immunofluorescence (IF) (15), Flow Cytometry (FACS) (13), ELISA (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunocytochemistry (ICC) (6), Enzyme Immunoassay (EIA) (5), Immunoprecipitation (IP) (3), Immunoelectron Microscopy (EM) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (1)
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Conjugates
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Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (2), internal (2), N-Term (1)
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