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Antibody
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anti-Protein Phosphatase 1 Regulatory Inhibitor Subunit 1B PPP1R1B
Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) antibody
| Antigen |
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| Synonyms |
Darpp32, AU040756, DARPP-32, DARPP32, FLJ20940, Darpp-32, darpp32, darpp-32, PPP1R1B |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Rat (Rattus) (41), Human (40), Mouse (Murine) (38), Cow (Bovine) (11), Chicken (9), Dog (Canine) (9), Zebrafish (Danio rerio) (3), Monkey (2), Primate (2), Rabbit (2), Xenopus laevis (2), Frog (1)
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
Western Blotting (WB) (27), Immunofluorescence (IF) (18), Flow Cytometry (FACS) (15), ELISA (6), Immunohistochemistry (IHC) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Enzyme Immunoassay (EIA) (3), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (EM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN361423 |
| Quantity |
100 µl (Variants) |
| Price |
345.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business days |
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Alternative name
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DARPP-32
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Format
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Affinity-purified
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Description
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DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002). Anti-DARPP-32 Western blot of a rat hippocampal lysate showing specific immunolabeling of the ~32k DARPP protein.
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Specificity
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Specific for the ~32k DARPP-32 protein.
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Application Notes
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Recommended Dilution: WB: 1:1000 IHC: 1:250 Quality Control: Western blots performed on each lot.
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Purification
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Prepared from rabbit serum by affinity purification via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.
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Storage
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Store at -20 °C, stable for 1 year
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Restrictions
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For Research Use only
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Alternatives for antigen "Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B)", type "Antibodies"
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Hosts
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Rabbit (42), Mouse (3)
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Reactivities
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Rat (Rattus) (41), Human (40), Mouse (Murine) (38), Cow (Bovine) (11), Chicken (9), Dog (Canine) (9), Zebrafish (Danio rerio) (3), Monkey (2), Primate (2), Rabbit (2), Xenopus laevis (2), Frog (1)
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Applications
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Western Blotting (WB) (27), Immunofluorescence (IF) (18), Flow Cytometry (FACS) (15), ELISA (6), Immunohistochemistry (IHC) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Enzyme Immunoassay (EIA) (3), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (EM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pThr75 (8), pThr34 (6), Ab-34 (1), Ab-75 (1), N-Term (1)
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