MAPK1/2 (C-Term) antibody

Catalog No. ABIN361430

Product Details

Target: MAPK1/2

Binding Specificity: C-Term

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for the ~42k - 44k ERK/MAPK protein. Immunolabeling is blocked by the peptide used as antigen.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: mouse

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References

Erickson, Ray, Wink, Bayon, Pedapati, Shaffer, Schaefer, Lahiri: "Initial analysis of peripheral lymphocytic extracellular signal related kinase activation in autism." in: Journal of psychiatric research, Vol. 84, pp. 153-160, (2017) (PubMed).

Target Details

Target: MAPK1/2

Background: Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002, Ahn, 1993, Tanoue and Nishida, 2003, Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993). Anti-ERK/MAPK Western blot of rat hippocampal homogenate showing specific immunolabeling of ~42-44k ERK/MAPK protein.

Molecular Weight: 42/44 kDa

Gene ID: 116590

UniProt: P63086