CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody

Details for Product No. ABIN361461
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Antigen
Synonyms creb, CREB1, Creb1, CREB, 2310001E10Rik, 3526402H21Rik, AV083133, Creb, Creb-1, creb1, zgc:55598
Epitope
pSer133
(75), (35), (27), (20), (19), (15), (15), (8), (8), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(363), (241), (229), (75), (69), (68), (45), (9), (6), (6), (5), (1), (1), (1), (1)
Host
Rabbit
(322), (54), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(11), (9), (9), (7), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2)
Application
Western Blotting (WB)
(283), (149), (147), (69), (60), (44), (30), (28), (13), (4), (2), (2), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 µL
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Catalog No. ABIN361461
379.50 $
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Specificity Specific for the ~45k CREB protein phosphorylated at Ser133. Immunolabeling is blocked by (-phosphatase treatment.
Purification Affinity-purified
Alternative Name CREB
Background It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999, Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004, Kornhauser et al., 2002). Anti-Phospho-Ser133 CREB Western blot of a forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45k CREB phosphorylated at Ser133 (Left Lane). The right lane is indicative that the specific immunolabeling of the CREB protein is completely eliminated by treatment with lambda phosphatase ((-Ptase, 1200 units for 30 min).
Application Notes Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
Restrictions For Research Use only
Format Liquid
Storage -20 °C
Supplier Images
anti-CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody Western blots of a forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45k CREB phosphorylated at Ser133 (Left Lane). The right lane is indicative that the specific immunolabeling of the CREB protein is completely eliminated by treatment with lambda phosphatase ((-Ptase, 1200 units for 30 min).
General Ahmed, Gardiner: "Preserving protein profiles in tissue samples: differing outcomes with and without heat stabilization." in: Journal of neuroscience methods, Vol. 196, Issue 1, pp. 99-106, 2011 (PubMed).

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