DARPP32 antibody (pThr75)

Catalog No. ABIN361463

There are 3+ publications for this product available. The Rabbit Polyclonal anti-DARPP32 antibody is suitable to detect DARPP32 in samples from Rat. It has been validated for WB and ELISA.

Quick Overview for DARPP32 antibody (pThr75) (ABIN361463)

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DARPP32 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DARPP32 Antibody

Binding Specificity: pThr75

Specificity: Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Thr75. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: bovine, canine, chicken, human, non-human primates

Characteristics: Our Anti-DARPP-32 (Thr75) Antibody phosphospecific primary antibody is rabbit polyclonal. It detects DARPP-32 (Thr75) and is antigen affinity purified. It is great for use in ELISA, WB.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 of rat DARPP-32 conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-DARPP32 antibody (ABIN361463)

Gomez, Midde, Mactutus, Booze, Zhu: "Environmental enrichment alters nicotine-mediated locomotor sensitization and phosphorylation of DARPP-32 and CREB in rat prefrontal cortex." in: PLoS ONE, Vol. 7, Issue 8, pp. e44149, (2013) (PubMed).

Cantrup, Sathanantham, Rushlow, Rajakumar: "Chronic hyperdopaminergic activity of schizophrenia is associated with increased ΔFosB levels and cdk-5 signaling in the nucleus accumbens." in: Neuroscience, Vol. 222, pp. 124-35, (2012) (PubMed).

Napolitano, Bonito-Oliva, Federici, Carta, Errico, Magara, Martella, Nistica: "Role of aberrant striatal dopamine D1 receptor/cAMP/protein kinase A/DARPP32 signaling in the paradoxical calming effect of amphetamine." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 33, pp. 11043-56, (2010) (PubMed).

Target Details for DARPP32

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Alternative Name: PPP1R1B

Background:

Synonyms: DARPP32 antibody, DARPP-32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody, Dopamine and cAMP regulated neuronal phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein antibody, Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody, Dopamine and cAMP regulated phosphoprotein DARPP32 antibody, Dopamine- and cAMP-regulated neuronal phosphoprotein antibody, FLJ20940 antibody, IPPD antibody, Neuronal phosphoprotein DARPP 32 antibody, PPP1R1B antibody, PPR1B_HUMAN antibody, Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody, Protein phosphatase 1 regulatory inhibitor subunit 1B antibody, Protein phosphatase 1 regulatory subunit 1B antibody

Description Logic: Our Anti-DARPP-32 (Thr75) Antibody phosphospecific primary antibody is rabbit polyclonal. It detects DARPP-32 (Thr75) and is antigen affinity purified. It is great for use in ELISA, WB.

Gene Name Alternatives: PPP1R1B

Molecular Weight: 32 kDa

Gene ID: 360616

UniProt: Q6J4I0