ATF2 (Phospho-Ser490,498) antibody

Antigen: ATF2 (Phospho-Ser490,498)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN361474

Additional Information

Format: Affinity-purified

Description: The activating transcription factor ATF2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (Maekawa et al., 1989). ATF2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF2 (Ivanov et al., 2003, Morton et al., 2004). Stress induced ATF-dependent transcription is dependent on phosphorylation of ATF (Fuchs et al., 2000), Morton et al., 2004). Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered an important substrate of signals upstream of the activation of genes associated with neuronal growth and differentiation (Karin and Hunter, 1995). ATF expression has also been linked to the depression in humans (Laifenfeld et al., 2004). Anti-Phospho Ser490,498 ATF2 Western blot of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immuno-labeling of the ~74k ATF2 protein phosphorylated at Ser490 and Ser498.

Specificity: Specific for ~74k ATF2 protein phosphorylated at Ser490,498. The antibody also recognizes the phosphorylated ~54k splice form of ATF2.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC (frozen sections, unpublished observations): 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immuno-labeling of the ~74k ATF2 protein phosphorylated at Ser490 and Ser498.

Publications

Publications: Chappell, Whalen, Gurai et al.: "Analysis of Flow Cytometry DNA Damage Response Protein Activation Kinetics after Exposure to X Rays and High-Energy Iron Nuclei." in: Radiation research, 2010 (PubMed). (1:1000).