Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1) (pSer831) antibody

Antigen: Glutamate Receptor, Ionotropic, AMPA 1 (GRIA1)

Synonyms: GLUH1, GLUR1, GLURA, HBGR1, MGC133252, Glr1, Glr-1, GluA1, GluRA, Glur1, HIPA1, GluR-A, Glur-1, AI853806, 2900051M01Rik, gluR-A, GRIA1, gria1

Epitope: pSer831

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN361476

Additional Information

Alternative name: Phospho-Ser831 GluR1

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser831 of GluR1.

Format: Affinity-purified

Description: Affinity purified rabbit polyclonal antibody. Biological Significance: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is potentiated by phosphorylation at Ser831 which has been shown to be mediated by either PKC or CaM kinase II (McGlade-McCulloh et al., 1993; Mammen et al., 1999; Roche et al., 1996). In addition, phosphorylation of this site has been linked to synaptic plasticity as well s learning and memory (Soderling and Derkach, 2000).

Specificity: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with canine, chicken, human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Specific for the ~100k GluR1 protein phosphorylated at Ser831. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is completely eliminated by λ-phosphatase treatment.

Application Details

Application Notes: WB: 1:1000

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Buffer: 100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg BSA per ml and 50% glycerol.

Storage: For long term storage –20°C is recommended. Stable at –20°C for at least 1 year.

Restrictions: For Research Use only

Images

Western blots of rat hippocampal lysate showing specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser831 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the GluR1 Ser831 antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Publications

Publications: Sears, Liu, Narayanan et al.: "Regulation of nucleus accumbens activity by the hypothalamic neuropeptide melanin-concentrating hormone." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 24, pp. 8263-73, 2010 (PubMed).

Ferrario, Loweth, Milovanovic et al.: "Distribution of AMPA receptor subunits and TARPs in synaptic and extrasynaptic membranes of the adult rat nucleus accumbens." in: Neuroscience letters, 2011 (PubMed).

Jiao, Jalan-Sakrikar, Robison et al.: "Characterization of a central Ca2+/calmodulin-dependent protein kinase IIalpha/beta binding domain in densin that selectively modulates glutamate receptor subunit phosphorylation." in: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 24806-18, 2011 (PubMed).