ELK1 (pSer383) antibody

Antigen: ELK1

Epitope: pSer383

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN361487

Additional Information

Alternative name: Phospho-Ser383 Elk-1

Format: Affinity-purified

Description: Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors (Hill and Treisman, 1995). Elk-1 is thought to be phosphorylated by MAP kinase at Ser383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1 (Li et al., 2003). Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus (Thiels et al., 2002). Anti-Phospho Ser383 Elk-1 Western blot of recombinant Elk-1 showing specific immunolabeling of the ~46k Elk-1 phosphorylated at Ser383 (Phos). The immunolabeling is absent in dephospho-Elk-1 (Non-Phos).

Specificity: Specific for ~46k Elk-1 phosphorylated at Ser383. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of recombinant Elk-1 showing specific immunolabeling of the ~46k Elk-1 phosphorylated at Ser383 (Phos). The immunolabeling is absent in dephospho-Elk-1 (Non-Phos).